GAb 2002

Final Programme

 Sunday 20 October
10.00 - 18.00 Registration (Lunch not provided)
Taxi or shuttle from the airport
14.00 - 14.15 Welcome/Introduction
Joachim Walter, Amersham Biosciences Membrane Separations, Boston, MA, USA
14.15 - 15.00  Keynote Address
"Protein A; From a Bacterial Cell Wall Protein to an Industrial Tool for Monoclonal Antibody GMP Production"
Mathias Uhlen, Royal Institute of Technology, Stockholm, Sweden
15.00 - 15.30 Break - Refreshments
Session I "A Family of GAbs; Success Stories"
Chair: Joachim Walter, Amersham Biosciences Membrane Separations, Boston, MA, USA
15.30 - 16.15 "Remicade Story: Rags to Riches"
Andrew Ramelmeier, -Centocor, Inc., Malvern, PA, USA
16.15 - 17.00 "Campath; Bringing a Therapeutic Monoclonal Antibody to Market"
Patrick Shannon, ILEX Oncology, San Antonio, TX, USA
18.00 - 19.00  Welcome Reception - with drinks and canapés
19.00 - 20.00  Poster Session I (presence of presenters not required)
Monday 21 October
07.30 - 10.30 Registration
07.00 - 08.30 Breakfast
Session II "Process Development and Design I"
Chair: Joerg Thommes, IDEC Pharmaceuticals, San Diego, CA, USA
08.30 - 09.00 Scale-Up of a Disc Stack Centrifuge for CHO Harvest
Jean Bender*, Arick Brown, and Charles Winter
Genentech, Inc. 1 DNA Way South San Francisco, CA 94080
09.00 - 09.30 Retentate Chromatography – Mass Spectrometry (RC-MS): A Novel Technique to Facilitate Development of Chromatographic Conditions for Antibody Purification
Patrick Santambien, Virginie Brenac , Warren Schwartz and Sylvio Bengio
Biosepra, Process division of Ciphergen – 48 avenue des Genottes F 95800 Cergy France
09.30 - 10.00 Evaluating Chromatographic Process Variability Using Design of Experiments
Christopher Bork*, Eric Cunningham, Deanna Myers, and Lynn Conley, IDEC Pharmaceuticals, Inc. 3010 Science Park Road, San Diego, CA 92121.USA
10.00 - 10.30 Break - Refreshments
10.30 - 11.00  Characterization and Optimization of Direct Capture of Monoclonal Antibodies on MAbSelect™
Tim Breece*1, Jennifer Silapie1, Annika Bergenstrahle2, Hans J Johansson3, and Charles Schmelzer1
1 Department of Recovery Sciences, Genentech Inc, 1 DNA Way, South San Francisco, CA 94080 USA
2 Amersham Biosciences, Bjorkgatan 30, SE-751 84 Uppsala, Sweden
3 Amersham Biosciences Corp., 800 Centennial Avenue, Piscataway, NJ 08855, USA
11.00 - 11.30 Design of Resins for Antibody Purification
Egbert Muller, TOSOH BIOSEP GmbH, Zettachring 6, D-70567 Stuttgart, Germany
Heiner Bottinger, Institut Fur Zellbiologie und Immunologie der Universitat Stuttgart, Allmandring 36, 70569 Stuttgart
12.00 - 15.30 Box lunch/Excursion
Medieval Guimaraes or Panoramic Tour of Porto
12.00 - 15.30 Alternative: Lunch at hotel and free time
16.00 - 17.30 Poster Session with refreshments
Session III "Process Development and Design II"
Chair: Andrew Ramelmeier, Centocor, Inc., Malvern, PA, USA
17.30 - 18.00 The Use of Anion Exchange Membrane Adsorbers for Contaminant Removal in Monoclonal and Recombinant Protein Production
Hélène PORA, Chris HALL and Sam NOCHUMSON, Pall Life Sciences
18.00 - 18.30 Development and Validation of a Virus Filtration Step for a Monoclonal Antibody Product
Authors: Charles Felice, Jennifer Reinert, Peter Wojciechowski*
Centocor Inc., Malvern PA, USA
18.30 - 19.00 Optimized Protein a Chromatography for Antibody Purification at 20kg/Batch Scale
Ping Y. Huang, Ph.D., Protein Design Labs, Inc.
20.00 - 20.15 Coach transfer to Graham's Port Cellar - Casual business attire
20.15 - 21.00 Cellar tour and port tasting
21.00 - 24.00 Banquet and entertainment
 Tuesday 22 October
07.00 - 08.00 Breakfast
Session IV "Therapeutic Antibodies of Modified Structure"
Chair: Steven Chamow, Abgenix, Inc., Fremont, CA, USA
08.00 - 08.30 Characterization of a F(ab')2 Fragment from a Human Immunoglobulin IgG2 Produced in Mammalian Cell Culture Fluid by Endogenous Enzymatic Digestion
Gerardo Zapata, Abgenix Inc., Fremont, CA, USA
08.30 - 09.00 Impacts of Glycosylation on Antibody Effector Function
Victor Sarantschin, Director, Technical Marketing, Neose Technologies, Inc.
09.00 - 09.30 Chromatographic Separation of Mannose- and Complex-type glycoforms of Monoclonal Antibodies
Markus Heitzmann* & Margit Jeschke, Novartis Pharma AG, Biotechnology Development, CH-4002 Basel
09.30 - 10.00 Break - Refreshments
10.00 - 10.30 Antibody-drug conjugates: Conferring therapeutic potency to Anti-tumor antibodies
Robert Lutz, ImmunoGen, Cambridge, MA, USA
10.30 - 11.00 Regulatory Expectations for Therapeutic GAbs
Gail Sofer, BioReliance, USA
11.00 - 11.15 Break
Session V "Design of Affinity Ligands"
Chair: Oguz Ersoy, Amersham Biosciences, Uppsala, Sweden
11.15 - 11.45 Stabilization of a staphylococcal protein A domain towards
alkaline conditions

Sophia Hober1*, Martin Linhult1, Susanne Gülich1, Anna Sjöberg2, Karin
Nord2 and Stefan Ståhl2
1Dept. of Biotechnology, Royal Institute of Technology, Stockholm, and
2Affibody AB, Bromma, Sweden.
11.45 - 12.15 Design and Study of a Specific Peptite Affinity Matrix for Selective
and Robust Mab Purification.
N. Ameskamp*, R. Frank(1), N. Sewald(2), J. Lehmann and D. Lütkemeyer
Institute of Cell Culture Technology, University of Bielefeld, Germany
(1) Molecular Recognition, GBF Braunschweig, Germany
(2) Organic Chemistry III, University of Bielefeld, Germany
 12.15 - 12.45 Protein L Mutants for the Purification ofAntibody and Antibody Fragments
Michael G. Gore*,2 Nicholas G. Housden 2 , Enrico A. Stura 1 and Marc Graille 1
1 CEA, Département d'Ingénierie et d'Etudes des Protéines (DIEP), C.E. Saclay, 91191 Gif-sur-Yvette Cedex, France
2 Department of Biochemistry, Institute of Biomolecular Sciences, University of Southampton, UK
12.45 - 13.15 Synthesis & Evaluation of a Protein L Mimic
Ana Cecilia Roque* 1,2, M. Angela Taipa1 and Christopher R. Lowe2
1 Centre for Biological and Chemical Engineering, Instituto Superior Técnico, Av. Rovisco Pais, 1049-001 Lisboa, Portugal
2 Institute of Biotechnology, University of Cambridge, Tennis Court Road,
Cambridge CB2 1QT, United Kingdom
13.15 - 13.30 Closing remarks
adjourn to lunch and departures