Final Programme of EBA02

Sunday, Sept 08, 2002
14.00-20.00 Registration Reception / Dinner / Welcome Address
Monday, Sept 09, 2002

Session 1 - Cell-Matrix interactions
Chairperson: Dr Joachim Walter, Boehringer Ingelheim Pharma KG, Germany
08.30-09.00 121 Influence of cells on hydrodynamics, thermodynamics, mass transfer and economics of expanded bed adsorption of proteins.
David Curbelo 1 *, Ruben Cuervo 1, Gunnar Garke 2, Wolf-Dieter Deckwer 3, F. Birger Anspach 4
(1) Centre of Molecular Immunology, P.O. Box 16040, Havana 11600, Cuba, (2)Schering AG, Germany, (3) GBF- Gesellschaft für Biotechnologische Forschung mbH, Biochemical Engineering Division, Mascheroder Weg 1, D-38124, Braunschweig, Germany, (4) PHARIS Biotec GmbH, Hannover, Germany
09.00-09.30 112 The influence of cell disruption conditions and surface charge on the Biomass Adsorbent Interactions in Expanded Beds
*Peter Brixius1, Dong-Quiang Lin3, Inger Mollerup2, Jürgen Hubbuch1, Maria-Regina Kula1
1 Institut of Enzymtechnologie, Heinrich-Heine Universität Düsseldorf, 52426 Jülich, Germany;
2 Novo Nordisk A/S; Protein Purification, Dept. 427, Hagedornsvej 1, 2820 Gentofte, Denmark,
3 Department of Chemical and Biochemical Engineering, Zhejiang University, 310027, Hangzhou, P. R. China
 09.30-10.00 103 The Use of Zeta Potential to Characterize Biomass/adsorbent Electrostatic Interactions in Expanded Bed
Dong-Qiang Lin *, Peter Brixius, Jürgen J. Hubbuch, Jörg Thömmes,
Maria-Regina Kula, Institut für Enzymtechnologie, Heinrich-Heine Universität Düsseldorf, im Forschungszentrum Jülich, 52426 Jülich, Germany
10.00-10.30 Coffee break

Session II - New Matrices
Chairperson: Prof. Dr. Howard Chase, University of Cambridge, UK
 10.30-11.00 123 Design of expanded bed adsorbents towards perfection.
Rolf Hjorth, Amersham Biosciences, Uppsala, Sweden
11.00-11.30 107 Evaluation of zirconia based ion exchanger for capturing recombinant endsotain from Pichia pastoris using expanded bed adsorption.
Patrick Santambien1, Egisto Boschetti1, Jim Spenser1, Loc Trinh2 and Joseph Shiloach2*.
1 Biospera, process division of ciphergen biosystems Cergy Saint Christophe, France
2 Biotechnology Unit NIH, NIDDK Bethesda, MD 20892 USA
11.30-12.00 120 Optimization of the EBA Performance by the Use of a Robust High Specific Peptide Ligand
Nicole Ameskamp(*), Ronald Frank (1), Norbert Sewald (2), Dirk Lütkemeyer, Jürgen Lehmann
Institute of Cell Culture Technology, University of Bielefeld, Germany
(1) Institute of Molecular Recognition, GBF Braunschweig, Germany
(2) Institute of Organic and Bioorganic Chemistry, University of Bielefeld, Germany
12.15-13.15 Buffet Lunch

Session III - Case Studies
Chairperson: Dr. Rhona O’Leary, Genentech Inc, USA
13.30-14.00 114 Isolation of Lactoferrin and Other Whey Proteins. Scaling-up from 1 to 150 cm Diameter Columns having a Rotating Distributing System.
Allan Lihme*, Ulrik Lund Jakobsen, Preben Kønigsfeldt+, Marie Bendix Hansen, UpFront Chromatography A/S, Lersø Parkallé 42 DK-2100 Copenhagen Ø, Denmark,; +Invensys APV, Pasteursvej, DK-8600 Silkeborg, Denmark.
14.00-14.30 131 Overcoming the Difficulties of Performing CEX-Expanded Bed Chromatography with Low pH E.coli Homogenates
Richard L. Gehant* and David W. Kahn
Department of Recovery Sciences, Genentech, Inc., 1 DNA Way, South San Francisco, CA 94080, USA
14.30-15.00 To be announced
18.00-19.30 Poster Session / Refreshments
19.30-22.00 Dinner
  Tuesday, Sept 10, 2002

Session IV - Engineering & Design
Chairperson: Mr Rolf Hjort, Amersham Biosciences, Sweden and Dr Jörg Thömmes, University of Düsseldorf, Germany
08.30-09.00 119 Scale-Flexible Fluid Distribution System for Expanded Bed Adsorption
Timothy J. Hobley*, Jürgen J. Hubbuch#, Anders Heebøll-Nielsen and Owen
R.T. Thomas
Center for Process Biotechnology, BioCentrum-DTU, Technical University of Denmark, Building 223, Søltofts Plads, DK-2800, Kgs. Lyngby, Denmark. #Present address: Institute of Enzyme Technology, Heinrich-Heine University, 52426 Jülich, Germany.
Jan Feuser*, Barbara Dietl, Daniel Buchholz, Ann-Kristin Barnfield Frej1, Mats Lundkvist1, Joachim K Walter
Boehringer Ingelheim Pharma KG, Biberach, Germany, 1Amersham Biosciences, Uppsala, Sweden
 09.30-10.00 102 Development of Buoyancy-Induced Mixing in Expanded Beds
Conan J. Fee*, Allan Neilsen, Jon Petersen, Michael Walmsley, Jens Bjerkwig, Lars Schultz,
Dept of Materials & Process Engineering, University of Waikato, Hamilton, New Zealand
10.00-10.30 Break
10.30-11.00 111 Modeling the Combined Effects of Particle Size Distribution
and Process Operational Parameters on the Performance of Expanded Bed Adsorption Process
Magnus Wistranda,b and Karol Lacki a*
a Amersham Biosciences AB, Uppsala, Sweden;
b Department of Molecular Biotechnology, Uppsala University, Uppsala, Sweden
11.00-11.30 To be announced
11.30-12.00 To be announced
12.15-13.15 Buffet Lunch / Box Lunch
13.15-17.30 Social Program
17.30-19.30 Poster Session / Refreshments
19.30-22.00 Dinner
  Wednesday, Sept 11, 2002

Session V - Novel Applications
Chairperson: Prof. Dr. Maria-Regina Kula, University of Düsseldorf, Germany
08.30-09.00 109 A novel process for continuous protein refolding and on-line EBA capture
Henrik Ferré*1, Owen R.T. Thomas1, Timothy J. Hobley1, Søren Buus2
1 Center for Process Biotechnology, BioCentrum-DTU, Technical University of Denmark, Building 223, Søltofts plads, DK-2800, Kgs. Lyngby, Denmark
2 Institute of Medical Microbiology and Immunology, Panum Institute, University of Copenhagen, Blegdamsvej 3, DK-2200, Copenhagen, Denmark
09.00-09.30 106 Application of Expanded Bed Adsorption Chromatography to Protein Recovery from Fusion Protein Cleavage
C. W. Suh, S. J. Nah, and E. K. Lee, Bioprocessing Research Laboratory
Department of Chemical Engineering, Hanyang University, Ansan, KOREA 425-791
09.30-10.00 To be announced
10.00-10.30  Break
10.30-11.00 116 EBA processing of potato fruit water on mixed mode adsorbent for functional protein recovery: A difficult separation task made possible.
Knut O. Strætkvern *, Hedmark College, Dept. of Agriculture and Natural Sciences, N-2322 Ridabu, Morten Aae Olander and Allan Lihme, UpFront Chromatography A/S, DK-2100 Copenhagen, Denmark
11.00-11.30 124 Cell Separations in Expanded Beds
Layth Ujam, Robert Clemmitt & Howard Chase*
Department of Chemical Engineering, University of Cambridge Pembroke Street, Cambridge CB2 3RA, U.K.
11.30-12.00 To be announced
12.00-12.30 Closing Remarks and Adjourn / Box Lunch